In this case report, it was described the histomorphology through optical microscopy of the implant-bone interface in an implant in function during 10 years, which had to be removed due to problems associated with the connection of the abutment. A 73-year-old male patient came asking for prosthesis mobility. Due the impossibility to replace the abutment it was decided to remove the implant with a 5 mm internal diameter trephine and analyze this sample histomorphologically. The histological analysis showed a mature trabecular tissue between the implant threads with a regular cell distribution and lamellar organization of calcified matrix, observing that bone tissue follows the shape of the implant’s surface with which it was in contact. Also, was observed low osteoclast activity, collagen type I, without signs of inflammation or resorption. In conclusion, implants have been shown in this case report, as well as by studies in humans to have long duration and outstanding biocompatibility that permits the formation of mature and regular peri-implant bone tissue in certain conditions.

A 73-year-old male patient without systemic diseases or negative health habits came asking about prosthesis mobility. At clinical examination, damage was identified in the inner thread of the posterior–superior endosteal implant. A review of his medical record indicated that the implant (Ankylos, Dentsply, New York, NY, USA) of 3.5 mm in diameter and 11 mm in length, had been in place for 10 years and 3 months. At the time of implantation, the bone level was optimal, so no ridge augmentation techniques were used. No pain, swelling, or implant mobility were observed. However, due the impossibility to replace the abutment it was decided to remove the implant with a 5 mm internal diameter trephine. The patient agreed with the suggested treatment and authorized through an informed consent the implant site extraction and histological examination of the sample. After removal of the implant, the resulting defect was filled with a biomaterial (DynaGraft-D putty, Keystone Dental, Burlington, MA, USA) for achieve bone tissue regeneration in order to schedule surgical placement of a new implant at a later date.

The sample was washed with PBS 1X 0.01M and then placed in a beaker with 300 mL of 10% ethylenediaminetetraacetic acid (EDTA) with 2% paraformaldehyde (pH 6.8) to partially decalcify and decrease the hardness of the bone tissue, generating a separation of the implant and bone tissue. Both solutions were changed every 6 hours for 15 days to obtain adequate decalcification. Under a stereoscopic microscope with 10X magnification, it was analyzed at the bone–implant interface at the level of the bottom of the most apical thread, where a thin metal probe was carefully inserted to separate the tissue carefully with a micro-incision metal clamp serrated 20 Ga to hold its shape. Once completely separating the bone tissue from the metal, it was immersed in EDTA to complete its decalcification. The tissue sample was cut 3 µm longitudinally with a microtome (Microm HM 325, Thermo Scientific, Florida, FL, USA) and subsequently stained using hematoxylineosin techniques, Picrosirius Red and Van Gieson’s stain. The samples were analyzed using an optical microscope (Olympus, Arquimed Innovation, Santiago, Chile) with magnification 4x, 10x and 50x. Histological analysis was performed on all sections obtained.

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